RNA Dynamics by NMR spectroscopy
M. Marušič, J. Schlagnitweit, K. Petzold, ChemBioChem 2019, 20, 2685.
An ever‐increasing number of functional RNAs require a mechanistic understanding. RNA function relies on changes in its structure, so‐called dynamics. To reveal dynamic processes and higher energy structures, new NMR methods have been developed to elucidate these dynamics in RNA with atomic resolution. In this Review, we provide an introduction to dynamics novices and an overview of methods that access most dynamic timescales, from picoseconds to hours. Examples are provided as well as insight into theory, data acquisition and analysis for these different methods. Using this broad spectrum of methodology, unprecedented detail and invisible structures have been obtained and are reviewed here. RNA, though often more complicated and therefore neglected, also provides a great system to study structural changes, as these RNA structural changes are more easily defined—Lego like—than in proteins, hence the numerous revelations of RNA excited states.
High-Resolution Field-Cycling NMR Studies of a DNA Octamer as a Probe of Phosphodiester Dynamics and Comparison with Computer Simulation
Mary F. Roberts, Qizhi Cui, Christopher J. Turner, David A. Case, and Alfred G. Redfield
Biochemistry 2004 43 (12), 3637-3650
Phosphorus-spin longitudinal relaxation rates of the DNA duplex octamer [d(GGAATTCC)]2 have been measured from 0.1 to 17.6 T by means of conventional and new field-cycling NMR methods. The high-resolution field-cycling method is identical to a conventional relaxation experiment, except that after preparation the sample is moved pneumatically from its usual position at the center of the high-resolution magnet upward to a lower field above its normal position and then returned to the center for readout after it has relaxed for the programmed relaxation delay at the low field. This is the first measurement of all longitudinal relaxation rates R1 of a nuclear species in a macromolecule over virtually the entire accessible magnetic field range....